MersV1, Main, Exploration, bibRecord, 004651

Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA

Identifieur interne : 004651 ( Main/Exploration ); précédent : 004650; suivant : 004652

Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA

Auteurs : M. L. Skotnicki [Australie] ; S. W. Ding [Australie] ; A. M. Mackenzie [Australie] ; A. J. Gibbs [Australie]

Source :

Archives of Virology [ 0304-8608 ] ; 1993-03-01.

RBID : ISTEX:2D30324D03D610AEEA4F7436DF6FB1A6BA7CFB57

English descriptors

Teeft :
- Cdna, Cdna clone, Cdna synthesis, Clone, Diii, Dsdna, Eggplant, Eggplant mosaic tymovirus, Eggplant mosaic virus, Encoding, Endonuclease, First strand primer, Genome, Genomic, Glutinosa, Glutinosa seedlings, Host range determination, Host range differences, Hybrid, Hydrolysed, Infectious clones, Inoculated, Italic, Ligated, Linearized, Molecular basis, Mosaic, Mosaic tymovirus, Mosaic virus, Nonviral, Nonviral nucleotides, Nucleic, Nucleic acids, Nucleotide, Nucleotide sequence, Ononis, Oymv, Oymv genome, Pbluescript, Plants inoculated, Plasmid, Polymerase, Primer, Restriction endonucleases, Seedling, Skotnicki, Smaller plasmids, States biochemicals, Systemic symptoms, Terminus, Turnip, Tymovirus, Tymoviruses, Tymv, Vector hydrolysed, Viral, Viral genome, Viral genomes, Viral sequence, Viral terminus, Virion, Virion protein gene, Virion protein genes.

Abstract

Summary: Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated toNicotiana glutinosa (for EMV) andPisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.

Url:

https://api.istex.fr/ark:/67375/1BB-JNNTVVNF-2/fulltext.pdf

DOI: 10.1007/BF01379079

Affiliations:

Australie

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Le document en format XML

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<term>Dsdna</term>
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<term>Eggplant mosaic tymovirus</term>
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<term>Ononis</term>
<term>Oymv</term>
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<term>Primer</term>
<term>Restriction endonucleases</term>
<term>Seedling</term>
<term>Skotnicki</term>
<term>Smaller plasmids</term>
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<term>Viral genomes</term>
<term>Viral sequence</term>
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<front><div type="abstract" xml:lang="en">Summary: Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated toNicotiana glutinosa (for EMV) andPisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in tymoviruses.</div>
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Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA

Infectious eggplant mosaic tymovirus and ononis yellow mosaic tymovirus from cloned cDNA

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri